| Origin | recombinant E. coli |
|---|---|
| Lineage name | Sarcosine : oxygen oxidoreductase (demethylating) |
| EC Number | 1.5.3.1 |
| Reaction formula | Sarcosine + H2O + O2 →→→ Glycine + Formaldehyde + H2O2 |
Sarcosine Oxidase (SOD-TE)
- The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
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SPECIFICATION
| Appearance | yellow lyophilizate | |
|---|---|---|
| Activity | ≧25 U/mg lyophilizate | |
| Contaminants | catalase <0.5% | |
| glucose oxidase <1.0×10-5％ | ||
| Stabilizer | sucrose | |
| Storage | below -20℃ protected from light |
PROPERTIES
| Molecular weight | ca. 49 kDa (gel filtration) |
|---|---|
| Structure | monomer of 43 kDa (SDS-PAGE) |
| one mole of FAD per mole of enzyme | |
| Isoelectric point | 5.3 |
| Michaelis constant | 4.7×10-3M (sarcosine) |
| pH Optimum | 6.7–9.5 |
| pH Stability | 6.5–10.5 |
| Optimum temperature | 50℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Zn2+，Cu2+，Hg2+，Ag+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
REFERENCES
- Suzuki, M., Medical Technology, 7, 945–950 (1979).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).
- Koyama, Y. et al., Agric. Biol. Chem., 55, 1259–1263 (1991)
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