Origin | recombinant E. coli |
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Lineage name | Creatine amidinohydrolase |
EC Number | 3.5.3.3 |
Reaction formula | Creatine + H2O →→→ Sarcosine + Urea |
SPECIFICATION
Appearance | white lyophilizate | |
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Activity | ≧9 U/mg lyophilizate | |
Contaminant | catalase <0.5% | |
Stabilizer | sucrose | |
Storage | below -20℃ |
PROPERTIES
Molecular weight | ca. 80 kDa (gel filtration) |
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Structure | 2 subunits of 46 kDa (SDS-PAGE) |
Michaelis constant | 1.3×10-2M (creatine) |
pH Optimum | 7.0–9.0 |
pH Stability | 5.0–11.0 |
Optimum temperature | 40℃ |
Thermal stability | below 45℃ |
Stability (liquid form) | stable at 37℃ for at least one month |
Stability (powder form) | stable at 30℃ for at least one month |
Inhibitors | Hg2+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
REFERENCES
- Suzuki, M, Medical Technology, 7, 945–950 (1979).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 140, 289–294 (1984).
- Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984).
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