| Origin | recombinant E. coli |
|---|---|
| Lineage name | Cholesterol : oxygen oxidoreductase |
| EC Number | 1.1.3.6 |
| Reaction formula | Cholesterol + O2 →→→ Cholest-4-en-3-one + H2O2 |
Cholesterol Oxidase (CHO-CE)
- The enzyme is useful for the determination of cholesterol in clinical analysis.
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SPECIFICATION
| Appearance | yellow lyophilizate | |
|---|---|---|
| Activity | ≧5 U/mg lyophilizate | |
| Contaminants | catalase ≦2.0% cholesterol esterase ≦1.0×10-2% | |
| Stabilizer | trehalose,glutamate, malate, EDTA | |
| Storage | below -20℃ protected from light |
PROPERTIES
| Molecular weight | ca. 58 kDa (gel filtration) |
|---|---|
| Structure | monomer of 55 kDa (SDS-PAGE) |
| Michaelis constant | 3.5×10-4 M (cholesterol) |
| pH Optimum | 7.0 |
| pH Stability | 5.0–10.0 |
| Optimum temperature | 50℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | ionic detergents, Ag+, Hg2+ |
APPLICATIONS
The enzyme is useful for the determination of cholesterol in clinical analysis.
REFERENCES
- Shirokane, Y. et al., J. Ferment. Technol., 55, 337–346 (1977).
- Richmond, W., Clin. Chem., 19, 1350–1356 (1973).
- Flegg, H. M., Ann. Clin. Biochem., 10, 79–84 (1973).
- Allain, C. C. et al., Clin. Chem., 20, 470–475 (1973).
- Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724–725 (1974).
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