| Origin | recombinant E. coli |
|---|---|
| Lineage name | Steryl-ester acylhydrolase |
| EC Number | 3.1.1.13 |
| Reaction formula | Cholesterol ester + H2O →→→→ Cholesterol + Fatty acid |
Cholesterol Esterase (CHE-XE)
- The enzyme is useful for the determination of cholesterol in clinical analysis.
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SPECIFICATION
| Appearance | light yellow lyophilizate | |
|---|---|---|
| Activity | ≧5 U/mg lyophilizate | |
| Stabilizer | sucrose | |
| Storage | below -20℃ |
PROPERTIES
| Molecular weight | ca. 54 kDa (gel filtration) |
|---|---|
| Structure | monomer of 54 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-5 M (cholesterol linoleate) |
| pH Optimum | 5.5–7.0 |
| pH Stability | 5.0–10.0 |
| Optimum temperature | 40℃ |
| Thermal stability | below 55℃ |
| Specificity | cholesterol linoleate (100), cholesterol acetate (2) cholesterol oleate (98), cholesterol palmitate (74) cholesterol stearate (68), cholesterol arachidonate (46) |
APPLICATIONS
The enzyme is useful for the determination of cholesterol in clinical analysis.
REFERENCES
- Richmond, W., Clin. Chem., 19, 1350 (1973)
- Flegg, H. M., Ann. Clin. Biochem., 10, 79 (1973).
- Alain, C. C. et al., Clin. Chem.,20, 470 (1973).
- Tarbutton, P. N. and Gunter, C. R., Clin. Chem., 20, 724 (1974).
- Kameno. Y. et al., Jap. J. Clin. Path., 24, 650 (1976).
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