| Origin | recombinant E. coli |
|---|---|
| Lineage name | Cholesterol : oxygen oxidoreductase |
| EC Number | 1.1.3 |
| Reaction formula | Cholesterol + 2O2 →→→ 6β-hydroperoxycholest-4-en-3-on + H2O2 |
Cholesterol Oxidase (CHO-PEWL)
- The enzyme is useful for the determination of cholesterol in clinical analysis.
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SPECIFICATION
| Appearance | liquid form (20–30% sucrose solution) | |
|---|---|---|
| Activity | ≧200 U/ml | |
| Storage | below -20℃ protected from light |
PROPERTIES
| Molecular weight | ca. 59 kDa (gel filtration) |
|---|---|
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-5 M (cholesterol) |
| pH Optimum | 6.5–8.0 |
| pH Stability | 3.5–8.5 |
| Optimum temperature | 55℃–65℃ |
| Thermal stability | below 70℃ |
| Stability (liquid form) | stable at 25℃ for at least one month |
| Inhibitors | Ag+, Hg2+ |
APPLICATIONS
The enzyme is useful for the determination of cholesterol in clinical analysis.
REFERENCES
- Aono, R. et al., Appl. Environ. Microbiol., 60, 2518–2523 (1994).
- Doukyu, N. et al., Appl. Environ. Microbiol., 64, 1929–1932 (1998).
- Doukyu, N. and Aono, R., Biochem. J., 341, 621–627 (1999).
- Doukyu, N. and Aono, R., Appl. Microbiol. Biotechnol., 57, 146–152 (2001).
- Aunpad, R. et al., Acta Cryst., D58, 2182–2183 (2002).
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