| Origin | recombinant E. coli |
|---|---|
| Lineage name | Maltose : orthophosphate 1-β-D-glucosyltransferase |
| EC Number | 2.4.1.8 |
| Reaction formula | Maltose + Orthophosphate →→→ D-Glucose + β-D-Glucose 1-phosphate |
Maltose Phosphorylase (MPL-EP)
- The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
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SPECIFICATION
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧10 U/mg lyophilizate | |
| Contaminants | NADH oxidase ≦5.0×10-4％ 6-phosphogluconate dehydrogenase ≦2.0×10-4％ | |
| Stabilizer | lactose,EDTA | |
| Storage | below -20℃ |
PROPERTIES
| Molecular weight | ca. 220 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 90 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-3M (phosphate) |
| 3.4×10-3 M (phosphate) | |
| 8.3×10-3M (arsenate) | |
| pH Optimum | 6.5–7.5 |
| pH Stability | 5.5–8.0 |
| Optimum temperature | 45–50°C |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least one week |
| Stability (powder form) | stable at 30℃ for at least four weeks |
| Inhibitors | Hg2+，Ag+，Zn2+，Cu2+ |
APPLICATIONS
The enzyme is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.
REFERENCES
- Hiruma, M. et al., Nippon Nogeikagaku Kaishi, 70, 773–780 (1996).
- Shirokane, Y. et al., Carbohydr. Res., 329, 699–702 (2000).
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