| Origin | calf intestinal mucosa |
|---|---|
| Lineage name | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) |
| EC Number | 3.1.3.1 |
| Reaction formula | An orthophosphoric monoester + H2O →→→ An alcohol + Orthophosphate |
Alkaline Phosphatase (ALP)
- The enzyme is widely used as a marker in ALP based immunoassay.
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SPECIFICATION
| Appearance | liquid form (50-60% glycerol solution) | |
|---|---|---|
| Activity | ≧6,000 U/mg protein | |
| Storage | between 2-7℃ (do not freeze) |
PROPERTIES
| Molecular weight | ca. 140 kDa (gel filtration) |
|---|---|
| pH Optimum | 9.5–10.0 |
| pH Stability | 6.5–11.0 |
| Optimum temperature | 50℃ |
| Thermal stability | below ca. 40℃ |
| Stability (liquid form) | stable at 37℃ for at least four weeks |
| Stability (liquid form) | stable at 5℃ for at least two years |
| Activators | Mg2+, Tris buffer, diethanolamine |
| Inhibitors | inorganic phosphate, EDTA |
APPLICATIONS
The enzyme is widely used as a marker in ALP based immunoassay.
REFERENCES
- O’Sullivan, M. J. et al., Anal. Biochem., 100, 100–108 (1979).
- Bergmeyer, H. U., “Methods of Enzymatic Analysis,” Vol. 2 (3rd ed.), Verlag Chemie, Weinheim, Germany, 1983, pp. 269–270.
- Crowther, J. R., “ELISA: Theory and Practice,” Humana Press, New York, 1995.
- Price, C. P. and Newman, D. J., “Principles and Practice of Immunoassay,” Macmillan Press, London, 1997.
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