SureFood® ALLERGEN ID Walnut
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The test detects Juglans regia (walnut) DNA according to directive (EC) 1169/2011 and Juglans nigra (eastern black walnut) DNA qualitatively and/or quantitatively. Each reaction contains an internal amplification control.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg walnut / kg food sample and a detection system including a standard dilution is required.
For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.
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General information:
The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
Specifications
| Art. No. | S3607 |
| Test format | 100 reactions |
| Detection limit | The SureFood® ALLERGEN Walnut PCR has a limit of detection of ≤ 0.4 mg / kg. |
Files
| Instructions | German/English |
