| Origin | recombinant E. coli |
|---|---|
| Lineage name | Urate : oxygen oxidoreductase |
| EC Number | 1.7.3.3 |
| Reaction formula | Urate + H2O + O2 →→→ Unidentified products + H2O2 ↓ CO2 + Allantoin (racemic mixture) |
Uricase (U-TE)
- The enzyme is useful for the determination of uric acid in clinical analysis.
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SPECIFICATION
| Appearance | light brownish lyophilizate | |
|---|---|---|
| Activity | ≧4U/mg lyophilizate | |
| Contaminant | catalase ≦1.0% | |
| Stabilizer | sucrose,citrate,EDTA | |
| Storage | below -20℃ |
PROPERTIES
| Molecular weight | ca. 90 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 35 kDa (SDS-PAGE) |
| Michaelis constant | 1.1×10-5M (uric acid) |
| pH Optimum | 8.5 |
| pH Stability | 7.0–11.0 |
| Optimum temperature | 45℃ |
| Thermal stability | below 55℃ |
| Stability (liquid form) | stable at 37℃ for at least ten days |
| Stability (powder form) | stable at 30℃ at least three weeks |
| Inhibitors | Hg2+，Ag+ |
APPLICATIONS
The enzyme is useful for the determination of uric acid in clinical analysis.
REFERENCES
Koyama, Y. et al., J. Biochem., 120, 969–973 (1996).
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