| Origin | recombinant E. coli |
|---|---|
| Lineage name | Fructosyl-peptide : oxygen oxidoreductase |
| EC Number | 1.5.3 |
| Reaction formula | Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2 |
Fructosyl-peptide Oxidase (FPOX-CET)
- The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
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SPECIFICATION
| Appearance | yellow lyophilizate | |
|---|---|---|
| Activity | ≧5.0 U/mg lyophilizate | |
| Stabilizer | glutamate, EDTA | |
| Storage | below -20℃ protected from light |
PROPERTIES
| Molecular weight | ca. 60 kDa (gel filtration) |
|---|---|
| Structure | monomer of 52 kDa(SDS-PAGE) |
| Michaelis constant | 1.5×10-3M(fructosyl-valyl-histidine) 5.0×10-3M (fructosyl-glysine) 9.0×10-3M(Nε-fructosyl-lysine) |
| pH Optimum | 7.5–8.5 |
| pH Stability | 5.5–9.5 |
| Optimum temperature | 37–45℃ |
| Thermal stability | below 45℃ |
| Stability (powder form) | stable at -20℃ for at least 12 months |
| Inhibitors | Hg2+ ，Pb2+ |
| Specificity | fructosyl-valyl-histidine (100), fructosyl-glycine(150) |
| Nε-fructosyl-L-lysine (68.6) |
APPLICATIONS
The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
REFERENCES
- Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104-111 (2003).
- Hirokawa, K. et al., Appl Microbiol Biotechnol., 78, 775-781 (2008).
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