Origin | recombinant E. coli |
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Lineage name | Fructosyl-peptide : oxygen oxidoreductase |
EC Number | 1.5.3 |
Reaction formula | Fructosyl-L-amino acid + H2O + O2 →→→ Peptide + Glucosone + H2O2 |
SPECIFICATION
Appearance | yellow lyophilizate | |
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Activity | ≧6.04 U/mg lyophilizate | |
Stabilizer | EDTA, glutamate | |
Storage | below -20℃ protected from light |
PROPERTIES
Molecular weight | ca. 60kDa (gel filtration) |
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Structure | monomer of 52 kDa (SDS-PAGE) |
Isoelectric point | 4.2 |
Michaelis constant | 3.4×10-4M (fructosyl-valyl-histidine) |
4.4×10-4M (fructosyl-glycine) | |
8.9×10-4M (Nε-fructosyl-lysine) | |
pH Optimum | 7.5–8.0 |
pH Stability | 6.0–9.5 |
Optimum temperature | 35–42℃ |
Thermal stability | below 45℃ |
Stability (powder form) | stable at 37℃ for at least one month |
Specificity | fructosyl-valyl-histidine (100), fructosyl-glycine (53) |
Nε-fructosyl-lysine (84) |
APPLICATIONS
The enzyme is useful for the determination of fructosyl-peptide and fructosyl-L-amino acid.
REFERENCES
- Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
- Hirokawa, K. et al., Arch. Microbiol., 180, 227–231 (2003).
- Hirokawa, K. et al., Biochem. Biophys. Res. Commun., 311, 104–111 (2003).
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