Reveal® for Histamine in Seafood | Neogen | AZ LAB
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Reveal® for Histamine in Seafood | Neogen | AZ LAB
Code: 9501
Manufacturer: Neogen - USA
Material provided:
+ 25 histamine test strips
+ 25 sample cups
+ 25 bottles of 7 mL sample diluent
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Suppliers
Reveal® for Histamine in seafood is a single-step lateral flow assay based on a competitive immunoassay format intended for the visual screening of histamine in scrombroid species of fish, such as tuna and mahi-mahi.
- Rapid 5 minutes test time
- Testing time: 5 minutes
- Antibody cross-reactivity: Specific for histamine
- Storage: Room temperature (18–30°C)
- Tests per kit: 25PROCEDURE HISTAMINE
How to take a fish sample (clean fish) cut at 3 positions with 1-inch long plate at the back of the pectoral fins, before the pelvic fins and the front of the fish's tail (in the illustration). Then put in the blender to homogenize the sample.
For further information about quotes or processes, please contact us for the most complete and accurate answer.
Features and Benefits
- Simple water extraction- Rapid 5 minutes test time
Product Specifications
- Sensitivity: 50 ppm- Testing time: 5 minutes
- Antibody cross-reactivity: Specific for histamine
- Storage: Room temperature (18–30°C)
- Tests per kit: 25
Results
PROCEDURE HISTAMINE
1. SAMPLE PREPARATION
How to take a fish sample (clean fish) cut at 3 positions with 1-inch long plate at the back of the pectoral fins, before the pelvic fins and the front of the fish's tail (in the illustration). Then put in the blender to homogenize the sample.- How to get a sample of canned food: mix the sample in the box.
- For other models please contact us for further advice.
- For other models please contact us for further advice.
2. EXPLOIT THE SAMPLE EXTRACT AND CHOOSE THE TEST LEVEL
According to research and empirical tests with companies that have used with the Reveal Histamine test we can test at concentrations: 100ppm, 50ppm, 25ppm and 16ppm on the Reveal Histamine test (code: 9501) by changing the procedure. extraction.
- Collect 10 g of homogeneous sample into extraction bottle.
- Add 190ml of distilled water to the extraction sample bottle.
- Cover tightly and shake bottle vigorously for 15 to 20 seconds.
- Wait about 5 minutes, then shake for 15-20 seconds.
- Wait another 5 minutes, and again shake the bottle from 15 to 20 seconds. Allow the tissue to settle to the bottom of the bottle for about 30 seconds. Ready-made samples for diluted extraction (1)
- Collect 10 g of homogeneous sample into extraction bottle.
- Add 190ml of distilled water to the extraction sample bottle.
- Cover tightly and shake bottle vigorously for 15 to 20 seconds.
- Wait about 5 minutes, then shake for 15-20 seconds.
- Wait another 5 minutes, and again shake the bottle from 15 to 20 seconds. Allow the tissue to settle to the bottom of the bottle for about 30 seconds. Ready-made samples for diluted extraction (1)
3.EXTRACTION, DILUTE
Because of the sensitivity of the assay, sample extracts must be diluted before performing the test. Using a clean suction tip, add 100 µl of diluted extract (1) to a 7ml (Sample Diluent) vial and close the lid and shake gently. The sample is ready for testing (2). Repeat the operation for other samples with a 7ml (Sample Diluent) vial with each sample. (Attention).
4. PROCESS
- Transfer the sample into the sample cup and place on the stand (or place on a flat position).
- Use a new tip and draw 200ul of the sample above (2) into the cup (3). Repeat for each other test piece with a new tip and a new cup (guaranteed cleaning).
- Place a new histamine test strip with the tip down into the beaker (3).
- Wait for 5 minutes for the sample to react with the test strip (note the time 5-6 minutes).
- Read visual results on a white background or Neogen Reveal AccuScan III Reader.
- Use a new tip and draw 200ul of the sample above (2) into the cup (3). Repeat for each other test piece with a new tip and a new cup (guaranteed cleaning).
- Place a new histamine test strip with the tip down into the beaker (3).
- Wait for 5 minutes for the sample to react with the test strip (note the time 5-6 minutes).
- Read visual results on a white background or Neogen Reveal AccuScan III Reader.
NOTES:
1 / Samples, when taken, must be stored in plastic or metal tools such as bottles, plastic bags, .. Do not use glassware to hold samples (as recommended by the manufacturer).
2 / Test Reveal Histamine (code: 9501) tested at 50ppm (standard). In addition, we can check at: 100ppm, 25ppm and 16ppm using the following diluted formula:
2 / Test Reveal Histamine (code: 9501) tested at 50ppm (standard). In addition, we can check at: 100ppm, 25ppm and 16ppm using the following diluted formula:
| Concentration | Extraction | Dilute the test sample (Add in 7ml extraction bottle) |
| 100ppm | 10g + 190ml distilled water | 50 µl sample, extract in bottle 7ml Diluent |
| 50ppm | 10g + 190ml distilled water | 100 µl sample, extract in bottle 7ml Diluent |
| 25ppm | 10g + 190ml distilled water | 200 µl sample, extract in bottle 7ml Diluent |
| 16ppm | 10g + 190ml distilled water | 300 µl sample, extract in bottle 7ml Diluent |
For further information about quotes or processes, please contact us for the most complete and accurate answer.
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