Sample Preparation
For indicator organisms (AQ, EC, ETC, and PA):
For indicator organisms (AQ, EC, ETC, and PA):
- For samples with very low microbial load (e.g potable water) – Filter 100ml of liquid sample using a 47mm membrane filter with 0.45µ pore size.
- For samples with low to high microbial load (e.g surface water and waste water) Directly inoculate 1ml of sample or if suspected to be high, dilute to 10-1 using sterile diluent.
For Pathogen (SL):
- Direct – Measure 100ml water sample and dilute with 100ml sterile double strength Buffered Peptone Water (BPW).
- Filtration – Filter 100ml water sample and transfer filter to 100ml sterile BPW.
Regulatory Limits
Refer to Word Health Oraganization (WHO), Environmental Protection Agency (EPA), or other local regulatory bodies.
Processing Notes
For chlorinated samples, make sure that the sterile container has enough sterile sodium thiosulfate (Na2S2O3) to neutralize chlorine in the water. For 100ml samples, add 100µl of 3% Na2S2O3 for potable water and 100µl of 10% Na2S2O3 for chlorinated waste or effluent water. Commercially prepared sampling containers usually have Na2S2O3 tablets.
Refer to Word Health Oraganization (WHO), Environmental Protection Agency (EPA), or other local regulatory bodies.
Processing Notes
For chlorinated samples, make sure that the sterile container has enough sterile sodium thiosulfate (Na2S2O3) to neutralize chlorine in the water. For 100ml samples, add 100µl of 3% Na2S2O3 for potable water and 100µl of 10% Na2S2O3 for chlorinated waste or effluent water. Commercially prepared sampling containers usually have Na2S2O3 tablets.
For filtered samples, add 1ml sterile diluent to rehydrate CompactDryTM plates. Transfer membrane filter using a rolling motion to avoid gas pockets between the filter and the fabric.
Similar to other tests that rely on β-Glucuronidase activity to detect E.Coli, CompactDryTM EC cannot detect E.Coli O157:H7 as an E.Coli.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
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