Sample Preparation
- For indicator organisms (TC, EC, ETB, YM, and XSA) – Cut out or weigh standardized sample size (e.g 10g or 25g) using sterile knife or scissors. Place in sterile container, then add appropriate volume of sterile diluent (e.g. 90ml or 225ml of Buffered Peptone Water or BPW, Maximum Recovery Diluent or MRD, or 0.1% Peptone Water). Homogenize and dilute accordingly.
- For pathogens (SL and LS) – Weigh 25g of sample and dilute with 225ml sterile BPW, the homogenize.
- For pathogen (VP) – Weigh 50g of sample and add 450ml of sterile Butterfield’s Phosphate Buffer (BPB), then homogenize. Use the surface tissues, gills, and gut for fish while for shellfish, use the meat and juice.
Refer to Codex Alimentarius, Food & Drug Administration (FDA), Food Standards.
Processing Notes
Raw seafood generally has high microbial load. For TC, plate using 10-3 (Figure 2) or 10-4 (Figure 3) dilution while using 10-1 for other indicator organisms. Cooked seafood tends to have lower microbial load. For TC, plate using 10-1 or 10-2.
Similar to other tests that rely on β-Glucuronidase activity to detect E.Coli, CompactDryTM EC cannot detect E.Coli O157:H7 as an E.Coli.
AZ LAB Co.,LTD is currently the official distributor of Nissui Compact Dry products in Vietnam with all the following criteria: Total Count (TC), E.coli and Colifrom (EC), Enterobacteriae (ETB), Vibrio parahaemolyticus (VP), Bacillus cereus (X-BC), Yeast & Mold (YM), Staphylococcus aureus (XSA), Enterococcus (ETC), Pseudomonas aeruginosa (PA), Salmonella (SL), and Listeria.
All information about Compact Dry products, customers can see at:
http://azlab.vn/en/shops/compact-dry/compact-dry-nissui-distributor-az-lab-in-vietnam.html
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