Origin | porcine heart |
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Lineage name | (S )-Lactate : NAD+ oxidoreductase |
EC Number | 1.1.1.27 |
Reaction formula | Pyruvate + NADH + H+ L-Lactate + NAD+ |
SPECIFICATION
Appearance | white lyophilizate | |
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Activity | ≧150 U/mg lyophilizate | |
Contaminants | GPT ≦1.0×10-2% | |
malate dehydrogenase ≦5.0×10-2% | ||
pyruvate kinase ≦1.0×10-2% | ||
Storage | below -20℃ |
PROPERTIES
Molecular weight | ca. 115 kDa (gel filtration) |
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Michaelis constant | 2.5×10-2M (fructosyl-glycine) 1.0×10-4M (pyruvate) |
pH Optimum | 7.4–8.0 |
pH Stability | 7.0–7.5 |
Optimum temperature | 50–55℃ |
Thermal stability | below 45℃ |
APPLICATIONS
The enzyme is useful for the determination of numerous metabolites (ATP, ADP, glucose, creatinine, pyruvate, lactate and glycerol) and of enzyme activities (GPT, PK and CPK) when coupled with the related enzymes.
REFERENCES
- Jaenicke, R. and Pfleiderer, G., Biochim. Biophys. Acta, 60, 615 (1962).
- Everse, J. and Kaplan, N. O., Adv. in Enzymol., 37, 61 (1973).
- Holbrook, J. J. et al., “The Enzyme,” Vol. 11 (3rd ed.), Academic Press, New York and London, 1975, pp. 191–292.
- Loshon, C. A. et al., Clin.Chem., 23, 1579 (1977).
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