Origin | recombinant E. coli |
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Lineage name | Creatinine amidohydrolase |
EC Number | 3.5.2.10 |
Reaction formula | Creatinine H2O Creatine |
SPECIFICATION
Appearance | yellow lyophilizate | |
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Activity | ≧500 U/mg lyophilizate | |
Contaminant | catalase ≦1.0% | |
Stabilizer | sucrose | |
Storage | below -20℃ |
PROPERTIES
Molecular weight | ca. 170 kDa (gel filtration) |
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Structure | 6 subunits of 28 kDa (SDS-PAGE) |
Isoelectric point | 4.8 |
Michaelis constant | 3.4×10-2M (creatinine) |
4.3×10-2M ((creatine) | |
pH Optimum | 6.5–7.0 |
pH Stability | 7.0–11.0 |
Optimum temperature | 60–65℃ |
Thermal stability | below 60℃ |
Stability (liquid form) | stable at 37°C for at least two weeks |
Stability (powder form) | stable at 30°C for at least one month |
Inhibitors | Hg2+ |
Activators | Mg2+,Mn2+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine in clinical analysis.
REFERENCES
Suzuki, M. and Yoshida, M., Clin. Chim. Acta, 143, 147–155 (1984)
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