| Origin | recombinant B.choshinensis |
|---|---|
| Lineage name | 3α-Hydroxysteroid : NAD(P)+ oxidoreductase |
| EC Number | 1.1.1.50 |
| Reaction formula | Androsterone + NAD(P)+ →→→ 5α-Androstane-3,17-dione + NAD(P)H |
3alpha-Hydroxysteroid Dehydrogenase (3aHSD-EH)
- The enzyme is useful for the determination of bile acids in clinical analysis.
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SPECIFICATION
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | ≧30 U/mg | |
| Stabilizer | trehalose | |
| Storage | below-20℃ |
PROPERTIES
| Molecular weight | ca. 41 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 25 kDa (SDS-PAGE) |
| Michaelis constant | 2.4×10-5M (androsterone) |
| 3.0×10-6M(NAD) | |
| pH Optimum | 11.0 |
| pH Stability | 6.0–10.0 |
| Optimum temperature | 50–60℃ |
| Thermal stability | below 45℃ |
| Stability (powder form) | stable at 37℃ for at least four weeks |
APPLICATIONS
The enzyme is useful for the determination of bile acids in clinical analysis.
REFERENCES
- Talalay, P., “The Enzymes,” Vol. 7 (2nd ed.), Academic Press, New York and London, 1963, pp. 177–202.
- Palmer, R., “Methods in Enzymology,” Vol. 15, Academic Press, New York and London, 1969, pp. 280–288.
- Möbus, E. and Maser, E., J. Biol. Chem., 273, 30888–30896 (1998).
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